Classical molecular dynamics simulations utilizing the Martini coarse-grained pressure subject had been carried out to review oil nanodroplets surrounded by fungal hydrophobin (HP) proteins in seawater. The class I EAS and the category II HFBII HPs had been studied together with two mannequin oils, specifically, benzene and n-decane. Both HPs exhibit free vitality minima on the oil-seawater interface, which is deeper in benzene in comparison with the n-decane techniques.
Larger constraint forces are required to maintain each HPs inside the n-decane part in comparison with inside benzene, with HFBII being extra affine to benzene in comparison with EAS. Smaller floor tensions are noticed at benzene-seawater interfaces coated with HPs in comparison with their n-decane counterparts. In the latter the floor rigidity stays unchanged upon will increase in the focus of HPs, whereas in benzene techniques including extra HPs result in decreases in floor rigidity.
EAS has a bigger tendency to cluster collectively in the interface in comparison with HFBII, with each HPs having bigger coordination numbers when surrounding benzene droplets in comparison with when they’re round n-decane nanoblobs. The HP-oil nanostructures in seawater examined have radii of gyration ranging between 2 and 12 nm, the place the n-decane buildings are bigger and have extra irregular shapes in comparison with the benzene techniques.
The n-decane molecules inside the nanostructures type a compact spherical core, with the HPs partially masking its floor and clustering collectively, conferring irregular shapes to the nanostructures. The EAS with n-decane buildings are bigger and have extra irregular shapes in comparison with their HFBII counterparts. In distinction, in the HP-benzene buildings each HPs are likely to penetrate the oil half of the droplet.
The HFBII-benzene buildings having the bigger oil/HP ratios examined are typically extra compact and spherical in comparison with their EAS counterparts; nonetheless, some of the HFBII-benzene techniques which have smaller oil/HP ratios have a extra elongated construction in comparison with their EAS counterparts. This simulation examine supplies insights into HP-oil nanostructures which are smaller than the oil droplets and fuel bubbles lately studied in experiments and, thus, could be difficult to look at with experimental methods.
A pure product of acteoside ameliorate kidney damage in diabetes db/db mice and HK-2 cells by way of regulating NADPH/oxidase-TGF-β/Smad signaling pathway
This examine was designed to research the protecting results and mechanisms of acteoside on DKD in diabetes male db/db mice and excessive glucose-induced HK-2 cells. The diabetes db/db mice had been divided randomly into mannequin group, metformin group, irbesartan group, and acteoside group. We noticed the pure product of acteoside exhibiting a major impact in renal safety by means of analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK-2 cells subjected to excessive glucose had been used in invitro experiments.
The molecular mechanisms of them had been investigated by RT-PCR and western blot. Acteoside prevents excessive glucose-induced HK-2 cells and diabetes db/db mice by inhibiting NADPH/oxidase-TGF-β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We found the pure product of acteoside exhibiting a major impact in renal safety. This examine paved the way in which for additional exploration of pathogenesis, early analysis, and growth of a brand new therapeutic agent for DKD.
Photophysical properties of heavy atom containing tetrasulfonyl phthalocyanines as doable photosensitizers in photodynamic remedy
The excitation energies, singlet-triplet vitality hole and spin-orbit coupling constants for Zn-, GaCl-, Pd-, and Pt- tetrasulfonyl phthalocyanines complexes (ZnComputer, GaClPc, PdComputer, and PtComputer) have been computed by utilizing the density purposeful idea and using the M06 exchange-correlation purposeful. Results present that these techniques possess attention-grabbing photophysical properties, which make them doable photosensitizers to be proposed in photodynamic remedy (PDT).
Absorption energies of all of the complexes examined have been discovered falling contained in the so-called therapeutic window (550-800 nm). Singlet-triplet vitality hole values are larger than these required for the manufacturing of cytotoxic molecular oxygen and the spin-orbit coupling constants are corresponding to to make sure an environment friendly spin orbit intersystem crossing. The obtained information are in step with the experimental oxygen singlet quantum yields. The platinum advanced seems to be the simplest candidate to suggest for PDT.
Purine adducts as a presumable lacking hyperlink for aristolochic acid nephropathy associated mobile vitality disaster, potential anti-fibrotic prevention and therapy
Aristolochic acid nephropathy (AAN) is a progressive exposome-induced illness characterised by tubular atrophy and fibrosis culminating in end-stage renal illness and malignancies. The molecular mechanisms of the vitality disaster as a putative trigger of fibrosis haven’t but been elucidated. In gentle of the truth that aristolochic acid (AA) varieties DNA and RNA adducts by covalent binding of AA metabolites to exocyclic amino teams of (deoxy)adenosine and (deoxy)guanosine, we hypothesize right here that related AA adducts might exist with different purine-containing molecules.
Casein, for Molecular Biology |
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| MB279-500G | EWC Diagnostics | 1 unit | EUR 387.65 |
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Description: Casein, for Molecular Biology |
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Urea for molecular biology, 99.5% |
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| 21113 | Sisco Laboratories | 500 Gms | EUR 6.5 |
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Description: Part A |
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Xylene for molecular biology, 99.5% |
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| 45122 | Sisco Laboratories | 250 ml | EUR 2.81 |
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Description: Part A |
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Acetone for molecular biology, 99.8% |
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| 27498 | Sisco Laboratories | 500 ml | EUR 3.76 |
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Description: Part A |
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Glycine for molecular biology, 99.5% |
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| 64072 | Sisco Laboratories | 100 Gms | EUR 1.64 |
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Description: Part A |
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Carbinol for molecular biology, 99.5% |
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| 34883 | Sisco Laboratories | 500 ml | EUR 6.23 |
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Description: Part A |
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Methanol for molecular biology, 99.5% |
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| 96446 | Sisco Laboratories | 500 ml | EUR 6.23 |
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Description: Part A |
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Formamide for molecular biology, 99.5% |
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| 30349 | Sisco Laboratories | 500 ml | EUR 5.82 |
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Description: Part A |
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Imidazole for molecular biology, 99.5% |
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| 61510 | Sisco Laboratories | 25 Gms | EUR 5.47 |
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Description: Part A |
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Chloroform for molecular biology, 99.8% |
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| 96764 | Sisco Laboratories | 100 ml | EUR 3.08 |
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Description: Part A |
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Urea, suitable for molecular biology |
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| GE1210 | Glentham Life Sciences | 500g | EUR 53.1 |
Urea, suitable for molecular biology |
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| GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
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| GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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| GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Urea, suitable for molecular biology |
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| GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
Triethylamine for molecular biology, 99.5% |
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| 55221 | Sisco Laboratories | 500 ml | EUR 4.11 |
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Description: Part A |
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Ficoll 400® for molecular biology |
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| 45460 | Sisco Laboratories | 5 Gms | EUR 15.6 |
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Description: Part B |
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Agarose UltraPhor for molecular biology |
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| 27813 | Sisco Laboratories | 10 Gms | EUR 46.5 |
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Description: Part B |
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Boric Acid for molecular biology, 99.5% |
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| 22311 | Sisco Laboratories | 250 Gms | EUR 4.04 |
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Description: Part A |
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2-Mercaptoethanol ?For Molecular Biology |
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| MB041-100ML | EWC Diagnostics | 1 unit | EUR 9.02 |
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Description: 2-Mercaptoethanol ?For Molecular Biology |
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2-Mercaptoethanol ?For Molecular Biology |
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| MB041-500ML | EWC Diagnostics | 1 unit | EUR 26.47 |
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Description: 2-Mercaptoethanol ?For Molecular Biology |
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ACES Buffer for molecular biology, 99.5% |
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| 27907 | Sisco Laboratories | 5 Gms | EUR 5.4 |
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Description: Part B |
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Triton X-100 for molecular biology |
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| 64518 | Sisco Laboratories | 100 ml | EUR 6.02 |
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Description: Part A |
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MOPS Buffer for molecular biology, 99% |
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| 66043 | Sisco Laboratories | 25 Gms | EUR 10.28 |
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Description: Part B |
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Vitamin B12 for molecular biology, 97% |
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| 77472 | Sisco Laboratories | 250 Mg | EUR 5.78 |
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Description: Part B |
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HEPES Buffer for molecular biology, 99.5% |
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| 16826 | Sisco Laboratories | 25 Gms | EUR 6.3 |
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Description: Part B |
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Sodium Azide for molecular biology, 99% |
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| 17782 | Sisco Laboratories | 100 Gms | EUR 5.47 |
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Description: Part A |
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CHAPS Buffer for molecular biology, 99.5% |
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| 21420 | Sisco Laboratories | 1 Gms | EUR 10.73 |
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Description: Part B |
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Agarose Low EEO for molecular biology |
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| 36601 | Sisco Laboratories | 10 Gms | EUR 5.1 |
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Description: Part B |
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PIPES Buffer for molecular biology, 99% |
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| 49159 | Sisco Laboratories | 100 Gms | EUR 26.03 |
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Description: Part B |
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10X MOPS Buffer for molecular biology |
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| 68078 | Sisco Laboratories | 100 ml | EUR 4.5 |
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Description: Part B |
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Agarose High EEO for molecular biology |
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| 60645 | Sisco Laboratories | 25 Gms | EUR 13.8 |
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Description: Part B |
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Jasmonic Acid for molecular biology, 95% |
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| 79238 | Sisco Laboratories | 25 Mg | EUR 120.9 |
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Description: Part B |
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Protamine Sulfate for molecular biology, 90-110% |
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| 78349 | Sisco Laboratories | 1 Gms | EUR 22.5 |
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Description: Part B |
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Oil Red O for molecular biology, 75% |
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| 23576 | Sisco Laboratories | 25 Gms | EUR 5.33 |
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Description: Part B |
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Agarose Medium EEO for molecular biology |
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| 10423 | Sisco Laboratories | 10 Gms | EUR 6.75 |
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Description: Part B |
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Sodium Chloride for molecular biology, 99.9% |
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| 33205 | Sisco Laboratories | 500 Gms | EUR 4.52 |
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Description: Part A |
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Isopropanol (IPA) for molecular biology, 99.8% |
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| 38445 | Sisco Laboratories | 100 ml | EUR 1.71 |
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Description: Part A |
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Agarose Low Melting for molecular biology |
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| 32417 | Sisco Laboratories | 5 Gms | EUR 22.5 |
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Description: Part B |
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Ammonium Acetate for molecular biology, 98% |
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| 37829 | Sisco Laboratories | 100 Gms | EUR 2.12 |
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Description: Part A |
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Acetonitrile (ACN) for molecular biology, 99.9% |
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| 62006 | Sisco Laboratories | 250 ml | EUR 4.93 |
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Description: Part A |
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Ethidium Bromide for molecular biology, 95% |
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| 93079 | Sisco Laboratories | 1 Gms | EUR 4.58 |
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Description: Part A |
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Ammonium Chloride for molecular biology, 99.5% |
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| 16992 | Sisco Laboratories | 500 Gms | EUR 4.52 |
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Description: Part A |
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Ammonium Sulphate for molecular biology, 99.5% |
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| 82126 | Sisco Laboratories | 250 Gms | EUR 4.93 |
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Description: Part A |
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Potassium Acetate for molecular biology, 99.5% |
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| 96248 | Sisco Laboratories | 100 Gms | EUR 2.74 |
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Description: Part A |
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Phenol Crystalline for molecular biology, 99.5% |
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| 17286 | Sisco Laboratories | 100 Gms | EUR 3.15 |
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Description: Part A |
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Sodium Bicarbonate for molecular biology, 99.7% |
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| 36328 | Sisco Laboratories | 500 Gms | EUR 5.47 |
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Description: Part A |
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Glycerol (Glycerine) for molecular biology, 99.5% |
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| 62417 | Sisco Laboratories | 100 ml | EUR 2.05 |
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Description: Part A |
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Potassium Chloride for molecular biology, 99.5% |
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| 84984 | Sisco Laboratories | 500 Gms | EUR 6.16 |
|
Description: Part A |
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Dichloromethane (DCM) for molecular biology, 99.9% |
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| 41512 | Sisco Laboratories | 250 ml | EUR 3.22 |
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Description: Part A |
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Acriflavine Neutral for molecular biology, 98% |
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| 42428 | Sisco Laboratories | 5 Gms | EUR 3.15 |
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Description: Part B |
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2-Mercaptoethanol for molecular biology, 99% |
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| 83759 | Sisco Laboratories | 100 ml | EUR 5.27 |
|
Description: Part A |
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Tetrahydrofuran (THF) for molecular biology, 99.8% |
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| 92562 | Sisco Laboratories | 250 ml | EUR 3.28 |
|
Description: Part A |
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Dithioerythritol (DTE) for molecular biology, 99% |
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| 53384 | Sisco Laboratories | 1 Gms | EUR 9.53 |
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Description: Part B |
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Sodium chloride, suitable for molecular biology |
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| GE0307 | Glentham Life Sciences | 1kg | EUR 41.33 |
Sodium chloride, suitable for molecular biology |
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| GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
L-Ascorbic Acid for molecular biology, 99.7% |
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| 14116 | Sisco Laboratories | 25 Gms | EUR 1.64 |
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Description: Part A |
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HEPES Sodium Salt for molecular biology, 99% |
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| 32225 | Sisco Laboratories | 25 Gms | EUR 11.85 |
|
Description: Part B |
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meta-Topolin (mT) for molecular biology, 98% |
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| 68913 | Sisco Laboratories | 25 Mg | EUR 17.48 |
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Description: Part B |
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Glycogen ex. Oyster for molecular biology, 85% |
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| 49740 | Sisco Laboratories | 1 Gms | EUR 7.5 |
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Description: Part B |
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Propidium Iodide (PI) for molecular biology, 95% |
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| 11195 | Sisco Laboratories | 10 Mg | EUR 17.63 |
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Description: Part B |
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Acetic Acid Glacial for molecular biology, 99.9% |
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| 59788 | Sisco Laboratories | 100 ml | EUR 3.56 |
|
Description: Part A |
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Acrylamide 3x cryst. for molecular biology, 99.9% |
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| 61346 | Sisco Laboratories | 100 Gms | EUR 5.47 |
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Description: Part A |
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Brilliant Blue G-250 for molecular biology |
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| 64222 | Sisco Laboratories | 5 Gms | EUR 4.65 |
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Description: Part B |
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Brilliant Blue R-250 for molecular biology |
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| 93473 | Sisco Laboratories | 5 Gms | EUR 4.35 |
|
Description: Part B |
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D-Sorbitol Powder for molecular biology, 98% |
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| 99103 | Sisco Laboratories | 500 Gms | EUR 10.33 |
|
Description: Part A |
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Acriflavine Hydrochloride for molecular biology, 98.5%-105% |
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| 35188 | Sisco Laboratories | 5 Gms | EUR 4.05 |
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Description: Part B |
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Tris Buffer Superior for molecular biology, 99.9% |
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| 37969 | Sisco Laboratories | 100 Gms | EUR 7.59 |
|
Description: Part A |
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Polysorbate 20 (Tween 20) for molecular biology |
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| 65296 | Sisco Laboratories | 100 Gms | EUR 10.81 |
|
Description: Part A |
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Molecular Biology Grade Water for RT-PCR |
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| ML065-1.5ML | EWC Diagnostics | 1 unit | EUR 7.82 |
|
Description: Molecular Biology Grade Water for RT-PCR |
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10X TE Buffer pH-8.0 for molecular biology |
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| 51782 | Sisco Laboratories | 100 ml | EUR 9.45 |
|
Description: Part B |
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10X TAE Buffer pH-8.3 for molecular biology |
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| 71989 | Sisco Laboratories | 100 ml | EUR 4.43 |
|
Description: Part B |
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Tris Buffer AR, ACS for molecular biology, 99.9% |
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| 71033 | Sisco Laboratories | 100 Gms | EUR 6.02 |
|
Description: Part A |
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10X TBE Buffer pH-8.3 for molecular biology |
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| 83170 | Sisco Laboratories | 200 ml | EUR 4.95 |
|
Description: Part B |
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MES Monohydrate Buffer for molecular biology, 99% |
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| 26854 | Sisco Laboratories | 25 Gms | EUR 11.03 |
|
Description: Part B |
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Acrylamide 40% aq. solution for molecular biology |
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| 29787 | Sisco Laboratories | 250 ml | EUR 20.53 |
|
Description: Part A |
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Glutathione Reduced (GSH) for molecular biology, 99.5% |
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| 11514 | Sisco Laboratories | 1 Gms | EUR 5.55 |
|
Description: Part B |
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DL-Dithiothreitol (DTT) for molecular biology, 98% |
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| 17315 | Sisco Laboratories | 1 Gms | EUR 5.18 |
|
Description: Part B |
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Sodium Acetate Anhydrous for molecular biology, 99% |
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| 22342 | Sisco Laboratories | 250 Gms | EUR 3.56 |
|
Description: Part A |
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Dimethyl Sulphoxide (DMSO) for molecular biology, 99.8% |
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| 24075 | Sisco Laboratories | 100 ml | EUR 6.02 |
|
Description: Part A |
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Trifluoroacetic Acid (TFA) for molecular biology, 99.9% |
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| 40801 | Sisco Laboratories | 100 ml | EUR 22.92 |
|
Description: Part A |
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Ammonium Persulphate (APS) for molecular biology, 99% |
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| 65553 | Sisco Laboratories | 25 Gms | EUR 1.64 |
|
Description: Part A |
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Glutathione Oxidized (GSSG) for molecular biology, 99.5% |
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| 22151 | Sisco Laboratories | 250 Mg | EUR 22.5 |
|
Description: Part B |
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Cesium Chloride ultrapure for molecular biology, 99.9% |
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| 32355 | Sisco Laboratories | 25 Gms | EUR 18 |
|
Description: Part B |
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Cesium Sulphate extrapure for molecular biology, 99.9% |
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| 57455 | Sisco Laboratories | 10 Gms | EUR 7.5 |
|
Description: Part B |
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Guanidine Thiocyanate (GTC) for molecular biology, 99% |
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| 80272 | Sisco Laboratories | 100 Gms | EUR 5.47 |
|
Description: Part A |
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Sodium Acetate Trihydrate for molecular biology, 99.5% |
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| 88035 | Sisco Laboratories | 250 Gms | EUR 2.33 |
|
Description: Part A |
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Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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| GS8780 | Glentham Life Sciences | 2500ml | EUR 239.63 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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| GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
Diethyl Pyrocarbonate (DEPC) for molecular biology, 99% |
|||
| 46791 | Sisco Laboratories | 5 ml | EUR 9 |
|
Description: Part B |
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Calcium Chloride Dihydrate for molecular biology, 99.5% |
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| 97080 | Sisco Laboratories | 100 Gms | EUR 2.12 |
|
Description: Part A |
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Pyridine, GlenBiol™, suitable for molecular biology |
|||
| GS6659 | Glentham Life Sciences | 500ml | EUR 230.38 |
Pyridine, GlenBiol™, suitable for molecular biology |
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| GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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| GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Formamide, GlenBiol™, suitable for molecular biology |
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| GS9663 | Glentham Life Sciences | 100ml | EUR 46.83 |
Formamide, GlenBiol™, suitable for molecular biology |
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| GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
Bromocresol Purple Sodium Salt for molecular biology |
|||
| 24836 | Sisco Laboratories | 5 Gms | EUR 2.05 |
|
Description: Part A |
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Guanidine Hydrochloride (GHC) for molecular biology, 99.5% |
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| 45539 | Sisco Laboratories | 100 Gms | EUR 4.79 |
|
Description: Part A |
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20X SSC Buffer pH 6.9-7.1 for molecular biology |
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| 12590 | Sisco Laboratories | 200 ml | EUR 2.48 |
|
Description: Part B |
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Polyethylene Glycol 8000 (PEG 8000) for molecular biology |
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| 54866 | Sisco Laboratories | 500 Gms | EUR 11.97 |
|
Description: Part A |
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10X Tris Buffered Saline (TBS) for molecular biology |
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| 83471 | Sisco Laboratories | 500 ml | EUR 14.7 |
|
Description: Part B |
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Magnesium Acetate Tetrahydrate for molecular biology, 99% |
|||
| 50488 | Sisco Laboratories | 100 Gms | EUR 2.74 |
|
Description: Part A |
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Magnesium Chloride Hexahydrate for molecular biology, 99.5% |
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| 91417 | Sisco Laboratories | 250 Gms | EUR 4.45 |
|
Description: Part A |
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Magnesium Sulphate Heptahydrate for molecular biology, 99.5% |
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| 74440 | Sisco Laboratories | 500 Gms | EUR 6.16 |
|
Description: Part A |
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Agarose Low EEO Superior Grade for molecular biology |
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| 23287 | Sisco Laboratories | 10 Gms | EUR 6 |
|
Description: Part B |
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L-Lysine Monohydrate (base) for molecular biology, 99% |
|||
| 45976 | Sisco Laboratories | 25 Gms | EUR 37.63 |
|
Description: Part A |
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Tris Hydrochloride (Tris HCl) for molecular biology, 99% |
|||
| 89781 | Sisco Laboratories | 100 Gms | EUR 9.37 |
|
Description: Part A |
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Cesium Chloride extrapure AR for molecular biology, 99.9% |
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| 22966 | Sisco Laboratories | 25 Gms | EUR 14.63 |
|
Description: Part B |
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N,N-Dimethylformamide (DMF) for molecular biology, 99.9% |
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| 24017 | Sisco Laboratories | 100 ml | EUR 3.08 |
|
Description: Part A |
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10X Tris-Tricine-SDS Buffer for molecular biology |
|||
| 37852 | Sisco Laboratories | 500 ml | EUR 9.08 |
|
Description: Part B |
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EDTA Disodium Salt Dihydrate for molecular biology, 99.5% |
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| 43272 | Sisco Laboratories | 100 Gms | EUR 2.26 |
|
Description: Part A |
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10X Tris-Glycine-SDS Buffer for molecular biology |
|||
| 57806 | Sisco Laboratories | 200 ml | EUR 3.6 |
|
Description: Part B |
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OORA00229-1L - Molecular Biology Grade UltraPure Water |
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| OORA00229-1L | Aviva Systems Biology | 1L | EUR 149 |
OORA00230-1L - Molecular Biology Grade UltraPure Water |
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| OORA00230-1L | Aviva Systems Biology | 1L | EUR 279 |
DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 243.6 |
NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 360 |
We additionally present new insights into the AA-induced vitality disaster and presumably a hyperlink between already recognized mechanisms. In addition, an summary of potential targets in fibrosis therapy is offered, which is adopted by suggestions on doable preventive measures that could possibly be taken to no less than postpone or partially alleviate AAN.
