Classical molecular dynamics simulations utilizing the Martini coarse-grained pressure subject had been carried out to review oil nanodroplets surrounded by fungal hydrophobin (HP) proteins in seawater. The class I EAS and the category II HFBII HPs had been studied together with two mannequin oils, specifically, benzene and n-decane. Both HPs exhibit free vitality minima on the oil-seawater interface, which is deeper in benzene in comparison with the n-decane techniques.
Larger constraint forces are required to maintain each HPs inside the n-decane part in comparison with inside benzene, with HFBII being extra affine to benzene in comparison with EAS. Smaller floor tensions are noticed at benzene-seawater interfaces coated with HPs in comparison with their n-decane counterparts. In the latter the floor rigidity stays unchanged upon will increase in the focus of HPs, whereas in benzene techniques including extra HPs result in decreases in floor rigidity.
EAS has a bigger tendency to cluster collectively in the interface in comparison with HFBII, with each HPs having bigger coordination numbers when surrounding benzene droplets in comparison with when they’re round n-decane nanoblobs. The HP-oil nanostructures in seawater examined have radii of gyration ranging between 2 and 12 nm, the place the n-decane buildings are bigger and have extra irregular shapes in comparison with the benzene techniques.
The n-decane molecules inside the nanostructures type a compact spherical core, with the HPs partially masking its floor and clustering collectively, conferring irregular shapes to the nanostructures. The EAS with n-decane buildings are bigger and have extra irregular shapes in comparison with their HFBII counterparts. In distinction, in the HP-benzene buildings each HPs are likely to penetrate the oil half of the droplet.
The HFBII-benzene buildings having the bigger oil/HP ratios examined are typically extra compact and spherical in comparison with their EAS counterparts; nonetheless, some of the HFBII-benzene techniques which have smaller oil/HP ratios have a extra elongated construction in comparison with their EAS counterparts. This simulation examine supplies insights into HP-oil nanostructures which are smaller than the oil droplets and fuel bubbles lately studied in experiments and, thus, could be difficult to look at with experimental methods.
A pure product of acteoside ameliorate kidney damage in diabetes db/db mice and HK-2 cells by way of regulating NADPH/oxidase-TGF-β/Smad signaling pathway
This examine was designed to research the protecting results and mechanisms of acteoside on DKD in diabetes male db/db mice and excessive glucose-induced HK-2 cells. The diabetes db/db mice had been divided randomly into mannequin group, metformin group, irbesartan group, and acteoside group. We noticed the pure product of acteoside exhibiting a major impact in renal safety by means of analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK-2 cells subjected to excessive glucose had been used in invitro experiments.
The molecular mechanisms of them had been investigated by RT-PCR and western blot. Acteoside prevents excessive glucose-induced HK-2 cells and diabetes db/db mice by inhibiting NADPH/oxidase-TGF-β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We found the pure product of acteoside exhibiting a major impact in renal safety. This examine paved the way in which for additional exploration of pathogenesis, early analysis, and growth of a brand new therapeutic agent for DKD.
Photophysical properties of heavy atom containing tetrasulfonyl phthalocyanines as doable photosensitizers in photodynamic remedy
The excitation energies, singlet-triplet vitality hole and spin-orbit coupling constants for Zn-, GaCl-, Pd-, and Pt- tetrasulfonyl phthalocyanines complexes (ZnComputer, GaClPc, PdComputer, and PtComputer) have been computed by utilizing the density purposeful idea and using the M06 exchange-correlation purposeful. Results present that these techniques possess attention-grabbing photophysical properties, which make them doable photosensitizers to be proposed in photodynamic remedy (PDT).
Absorption energies of all of the complexes examined have been discovered falling contained in the so-called therapeutic window (550-800 nm). Singlet-triplet vitality hole values are larger than these required for the manufacturing of cytotoxic molecular oxygen and the spin-orbit coupling constants are corresponding to to make sure an environment friendly spin orbit intersystem crossing. The obtained information are in step with the experimental oxygen singlet quantum yields. The platinum advanced seems to be the simplest candidate to suggest for PDT.
Purine adducts as a presumable lacking hyperlink for aristolochic acid nephropathy associated mobile vitality disaster, potential anti-fibrotic prevention and therapy
Aristolochic acid nephropathy (AAN) is a progressive exposome-induced illness characterised by tubular atrophy and fibrosis culminating in end-stage renal illness and malignancies. The molecular mechanisms of the vitality disaster as a putative trigger of fibrosis haven’t but been elucidated. In gentle of the truth that aristolochic acid (AA) varieties DNA and RNA adducts by covalent binding of AA metabolites to exocyclic amino teams of (deoxy)adenosine and (deoxy)guanosine, we hypothesize right here that related AA adducts might exist with different purine-containing molecules.
Urea, suitable for molecular biology |
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| GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Sodium chloride, suitable for molecular biology |
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| GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 360 |
DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 243.6 |
Tris (Molecular Biology Grade) |
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| CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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| CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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| CE239 | GeneOn | 5 kg | EUR 535.2 |
BCIP (Molecular Biology Grade) |
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| CE108 | GeneOn | 250 mg | EUR 75.6 |
BCIP (Molecular Biology Grade) |
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| CE109 | GeneOn | 1 g | EUR 108 |
DAPI (Molecular Biology Grade) |
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| CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
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| CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
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| CE119 | GeneOn | 100 mg | EUR 382.8 |
100mL Molecular Biology Grade |
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| 46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 74.4 |
500mL Molecular Biology Grade |
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| 46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 138 |
HEPES (Molecular Biology Grade) |
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| CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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| CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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| CE173 | GeneOn | 1 kg | EUR 424.8 |
Water (Molecular Biology Grade) |
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| CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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| CE244 | GeneOn | 1 l | EUR 67.2 |
CHAPS (Molecular Biology Grade) |
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| CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
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| CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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| CE116 | GeneOn | 25 g | EUR 492 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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| GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
Tween20 (Molecular Biology Grade) |
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| CE242 | GeneOn | 1 l | EUR 106.8 |
Glycine (Molecular Biology Grade) |
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| CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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| CE159 | GeneOn | 5 kg | EUR 228 |
Lysozyme (Molecular Biology Grade) |
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| CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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| CE189 | GeneOn | 10 g | EUR 247.2 |
Pyridine, GlenBiol™, suitable for molecular biology |
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| GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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| GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Formamide, GlenBiol™, suitable for molecular biology |
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| GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
1L Molecular Biology Grade Water |
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| 46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 196.8 |
Tween 20, Molecular Biology Grade |
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| T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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| T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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| T9100-100 | GenDepot | 1L | EUR 160.8 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
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| GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
MOPS buffer (Molecular Biology Grade) |
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| CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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| CE195 | GeneOn | 250 g | EUR 169.2 |
Glycerol 87 % (Molecular Biology Grade) |
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| CE154 | GeneOn | 1 l | EUR 93.6 |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
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| GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
Formamide deionized (Molecular Biology Grade) |
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| CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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| CE146 | GeneOn | 1 l | EUR 120 |
TritonX-100 (Molecular Biology Grade) |
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| CE240 | GeneOn | 500 ml | EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
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| CE241 | GeneOn | 1 l | EUR 79.2 |
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE121 | GeneOn | 500 ml | EUR 110.4 |
Dimethylformamide, GlenBiol™, suitable for molecular biology |
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| GS6580-2500 | Glentham Life Sciences | 2500 | EUR 107.3 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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| GK8512-1L | Glentham Life Sciences | 1 l | EUR 92.4 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
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| GS0247-1 | Glentham Life Sciences | 1 | EUR 40.7 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
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| GS0247-2500 | Glentham Life Sciences | 2500 | EUR 61.3 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
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| GS0969-1 | Glentham Life Sciences | 1 | EUR 47 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
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| GS0969-2500 | Glentham Life Sciences | 2500 | EUR 73.9 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
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| GS8649-1 | Glentham Life Sciences | 1 | EUR 43.1 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
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| GS8649-2500 | Glentham Life Sciences | 2500 | EUR 66.5 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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| GK8512-1 | Glentham Life Sciences | 1 | EUR 60.1 |
Water, Ultrapure Molecular Biology Grade |
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| 41024-4L | Biotium | 4L | EUR 145.2 |
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Description: Minimum order quantity: 1 unit of 4L |
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Sodium chloride (Molecular Biology Grade) |
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| CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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| CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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| CE207 | GeneOn | 5 kg | EUR 123.6 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE111 | GeneOn | 250 g | EUR 259.2 |
Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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| PD0252 | Bio Basic | 500g | EUR 192.59 |
Ammonia solution, GlenBiol™, suitable for molecular biology |
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| GS8853-100 | Glentham Life Sciences | 100 | EUR 91.2 |
Urea Crystalline (Molecular Biology Grade) |
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| CE167 | GeneOn | 1 kg | EUR 72 |
Urea Crystalline (Molecular Biology Grade) |
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| CE168 | GeneOn | 5 kg | EUR 181.2 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE105 | GeneOn | 250 g | EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE106 | GeneOn | 1 kg | EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE107 | GeneOn | 5 kg | EUR 153.6 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
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| GE6258-100 | Glentham Life Sciences | 100 | EUR 150.4 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
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| GE6258-25 | Glentham Life Sciences | 25 | EUR 60.1 |
SSC Buffer (20X) (Molecular Biology Grade) |
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| CE229 | GeneOn | 1 l | EUR 86.4 |
XTT sodium salt (Molecular Biology Grade) |
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| CE250 | GeneOn | 100 mg | EUR 208.8 |
XTT sodium salt (Molecular Biology Grade) |
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| CE251 | GeneOn | 500 mg | EUR 612 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE234 | GeneOn | 250 g | EUR 99.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE235 | GeneOn | 500 g | EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE236 | GeneOn | 1 kg | EUR 223.2 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE155 | GeneOn | 500 ml | EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE156 | GeneOn | 1 l | EUR 102 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE157 | GeneOn | 2.5 l | EUR 170.4 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE200 | GeneOn | 250 mg | EUR 92.4 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE201 | GeneOn | 1 g | EUR 190.8 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE164 | GeneOn | 100 g | EUR 86.4 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE165 | GeneOn | 500 g | EUR 192 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE166 | GeneOn | 1 kg | EUR 307.2 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE198 | GeneOn | 1 g | EUR 91.2 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE199 | GeneOn | 5 g | EUR 244.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE163 | GeneOn | 1 kg | EUR 352.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE160 | GeneOn | 100 g | EUR 93.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE161 | GeneOn | 250 g | EUR 153.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE162 | GeneOn | 500 g | EUR 232.8 |
Yeast extract powder (Molecular Biology Grade) |
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| CE169 | GeneOn | 500 g | EUR 133.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE148 | GeneOn | 500 g | EUR 67.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE149 | GeneOn | 1 kg | EUR 75.6 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE150 | GeneOn | 5 kg | EUR 180 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE174 | GeneOn | 1 g | EUR 232.8 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE175 | GeneOn | 5 g | EUR 920.4 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE204 | GeneOn | 500 mg | EUR 374.4 |
3-Methylbutan-1-ol, GlenBiol™, suitable for molecular biology |
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| GS6015-100 | Glentham Life Sciences | 100 | EUR 74.5 |
3-Methylbutan-1-ol, GlenBiol™, suitable for molecular biology |
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| GS6015-500 | Glentham Life Sciences | 500 | EUR 301.4 |
TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology |
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| GE6710-1 | Glentham Life Sciences | 1 | EUR 67.9 |
TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology |
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| GE6710-250 | Glentham Life Sciences | 250 | EUR 22.7 |
Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology |
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| GS7181-100 | Glentham Life Sciences | 100 | EUR 143.3 |
Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology |
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| GS7181-500 | Glentham Life Sciences | 500 | EUR 474.2 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE100 | GeneOn | 50 g | EUR 128.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE101 | GeneOn | 100 g | EUR 193.2 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE102 | GeneOn | 250 g | EUR 387.6 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE103 | GeneOn | 500 g | EUR 656.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE104 | GeneOn | 1 kg | EUR 1162.8 |
Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0) |
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| GX7489-1 | Glentham Life Sciences | 1 | EUR 22.7 |
Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0) |
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| GX7489-2500 | Glentham Life Sciences | 2500 | EUR 45.2 |
Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0) |
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| GX7489-500 | Glentham Life Sciences | 500 | EUR 15.9 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-193 | Genesee Scientific | 100ml/Unit | EUR 268 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-194 | Genesee Scientific | 500ml/Unit | EUR 294 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-195 | Genesee Scientific | 1000ml/Unit | EUR 308 |
We additionally present new insights into the AA-induced vitality disaster and presumably a hyperlink between already recognized mechanisms. In addition, an summary of potential targets in fibrosis therapy is offered, which is adopted by suggestions on doable preventive measures that could possibly be taken to no less than postpone or partially alleviate AAN.
