In this research, the molecular mechanisms through which Mitochondrial Ribosomal Protein S17 (MRPS17) contributes to gastric cancer (GC) and its prognostic significance in GC have been explored. As a protein encoding gene, MRPS17 encodes a 28s proteins belonging the ribosomal protein S17P household. The particular roles and molecular mechanisms of MRPS17 in cancers stay ambiguous. It was revealed by analyzing information from TCGA and GEO that elevated expression of MRPS17 was considerably related to invasion of GC and poor survival of GC sufferers.
Then through univariate and multivariate Cox regression analyses it was demonstrated that MRPS17 an unbiased prognostic issue for GC sufferers (P<0.001). It was demonstrated by differentially expressed gene evaluation and purposeful enrichment evaluation that MPRS17 is expounded to PI3K/AKT pathway and Cell adhesion molecules (CAMs), whereas its operate is mediated by collagen-containing extracellular matrix and receptor ligand/regulator exercise.
Then it was confirmed by in-vitro experiments that pulling down of MRPS17 gene in AGS and SGC7901 cells would considerably inhibit proliferation and invasion functionality of those cells. Furthermore, it was revealed by cell immunofluorescence assay that as a ribosomalprotein, MRPS17 was primarily distributed within the cytoplasmic floor of cell membrane.
Additionally, activation of PI3K/AKT pathway is accountable for malignant development of glioma that was promoted by MRPS17. In conclusion, it was revealed within the current research that MRPS17 promoted invasion and metastasis of GC and potential molecular mechanisms through which it exerted its influences on GC had been explored, suggesting its potential as a novel prognostic biomarker for GC.
Molecular Characterization of Superficial Layers of the Presubiculum During Development
The presubiculum, a subarea of the parahippocampal area, performs a essential function in spatial navigation and spatial illustration. An excellent facet of presubicular spatial codes is head-direction selectivity of the firing of excitatory neurons, known as head-direction cells. Head-direction selectivity emerges earlier than eye-opening in rodents and is maintained in maturity through neurophysiological interactions between excitatory and inhibitory neurons.
Although the presubiculum has been physiologically profiled when it comes to spatial illustration throughout improvement, the histological traits of the growing presubiculum are poorly understood. We discovered that the expression of vesicular glutamate transporter 2 (VGluT2) could be used to delimit the superficial layers of the presubiculum, which was recognized utilizing an anterograde tracer injected into the anterior thalamic nucleus (ATN).
Thus, we immunostained slices from mice ranging in age from neonates to adults utilizing an antibody towards VGluT2 to judge the VGluT2-positive space, which was recognized because the superficial layers of the presubiculum, throughout improvement. We additionally immunostained the slices utilizing antibodies towards parvalbumin (PV) and somatostatin (SOM) and discovered that within the presubicular superficial layers, PV-positive neurons progressively elevated in quantity throughout improvement, whereas SOM-positive neurons exhibited no rising development.
In addition, we noticed repeating patch buildings in presubicular layer III from postnatal days 12. The considerable expression of VGluT2 means that the presubicular superficial layers are regulated primarily by VGluT2-mediated excitatory neurotransmission. Moreover, developmental modifications within the densities of PV- and SOM-positive interneurons and the emergence of the VGluT2-positive patch buildings throughout adolescence could be related to the purposeful improvement of spatial codes within the superficial layers of the presubiculum.
Rice Seedling Growth Promotion by Biochar Varies With Genotypes and Application Dosages
While biochar use in agriculture is broadly advocated, how the impact of biochar on plant development varies with biochar varieties and crop genotypes is poorly addressed. The function of dissolvable natural matter (DOM) in plant development has been more and more addressed for crop manufacturing with biochar. In this research, a hydroponic tradition of rice seedling development of two cultivars was handled with bulk mass (DOM-containing), water extract (DOM solely), and extracted residue (DOM-free) of maize residue biochar, at a volumetric dosage of 0.01, 0.05, and 0.1%, respectively.
On seedling root development of the 2 cultivars, bulk biochar exerted a usually unfavorable impact, whereas the biochar extract had a constantly constructive impact throughout the appliance dosages. Differently, the extracted biochar confirmed a contrasting impact between genotypes. In one other hydroponic tradition with Wuyunjing 7 handled with biochar extract at sequential dosages, seedling development was promoted by 95% at 0.01% dosage however by 26% at 0.1% dosage, defined with the nice promotion of secondary roots slightly than of main roots.
Such results had been possible defined by low molecular weight natural acids and nanoparticles contained within the biochar DOM. This research highlights the significance of biochar DOM and crop genotype when evaluating the impact of biochar on crops. The use of low dosage of biochar DOM could assist farmers to undertake biochar expertise as an answer for agricultural sustainability.
The Inhibitory Receptor CLEC12A Regulates PI3K-Akt Signaling to Inhibit Neutrophil Activation and Cytokine Release
The myeloid inhibitory C-type lectin receptor CLEC12A limits neutrophil activation, pro-inflammatory pathways and illness in mouse fashions of inflammatory arthritis by a molecular mechanism that continues to be poorly understood. We addressed how CLEC12A-mediated inhibitory signaling counteracts activating signaling by cross-linking CLEC12A in human neutrophils. CLEC12A cross-linking induced its translocation to flotillin-rich membrane domains the place its ITIM was phosphorylated in a Src-dependent method.
Phosphoproteomic evaluation recognized candidate signaling molecules regulated by CLEC12A that embrace MAPKs, phosphoinositol kinases and members of the JAK-STAT pathway. Stimulating neutrophils with uric acid crystals, the etiological agent of gout, drove the hyperphosphorylation of p38 and Akt.
CHAPS (Molecular Biology Grade) |
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| CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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| CE116 | GeneOn | 25 g | EUR 492 |
DAPI (Molecular Biology Grade) |
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| CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
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| CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
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| CE119 | GeneOn | 100 mg | EUR 382.8 |
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE121 | GeneOn | 500 ml | EUR 110.4 |
DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 243.6 |
Glycine (Molecular Biology Grade) |
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| CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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| CE159 | GeneOn | 5 kg | EUR 228 |
HEPES (Molecular Biology Grade) |
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| CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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| CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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| CE173 | GeneOn | 1 kg | EUR 424.8 |
Lysozyme (Molecular Biology Grade) |
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| CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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| CE189 | GeneOn | 10 g | EUR 247.2 |
NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 360 |
Tris (Molecular Biology Grade) |
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| CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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| CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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| CE239 | GeneOn | 5 kg | EUR 535.2 |
Tween20 (Molecular Biology Grade) |
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| CE242 | GeneOn | 1 l | EUR 106.8 |
Water (Molecular Biology Grade) |
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| CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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| CE244 | GeneOn | 1 l | EUR 67.2 |
100mL Molecular Biology Grade |
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| 46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 70.68 |
500mL Molecular Biology Grade |
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| 46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 124.26 |
Water, Ultrapure Molecular Biology Grade |
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| 41024-4L | Biotium | 4L | EUR 145.2 |
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Description: Minimum order quantity: 1 unit of 4L |
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Ammonium sulfate (Molecular Biology Grade) |
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| CE105 | GeneOn | 250 g | EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE106 | GeneOn | 1 kg | EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE107 | GeneOn | 5 kg | EUR 153.6 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE111 | GeneOn | 250 g | EUR 259.2 |
Formamide deionized (Molecular Biology Grade) |
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| CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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| CE146 | GeneOn | 1 l | EUR 120 |
Glycerol 87 % (Molecular Biology Grade) |
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| CE154 | GeneOn | 1 l | EUR 93.6 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE155 | GeneOn | 500 ml | EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE156 | GeneOn | 1 l | EUR 102 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE157 | GeneOn | 2.5 l | EUR 170.4 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE160 | GeneOn | 100 g | EUR 93.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE161 | GeneOn | 250 g | EUR 153.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE162 | GeneOn | 500 g | EUR 232.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE163 | GeneOn | 1 kg | EUR 352.8 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE164 | GeneOn | 100 g | EUR 86.4 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE165 | GeneOn | 500 g | EUR 192 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE166 | GeneOn | 1 kg | EUR 307.2 |
Urea Crystalline (Molecular Biology Grade) |
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| CE167 | GeneOn | 1 kg | EUR 72 |
Urea Crystalline (Molecular Biology Grade) |
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| CE168 | GeneOn | 5 kg | EUR 181.2 |
MOPS buffer (Molecular Biology Grade) |
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| CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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| CE195 | GeneOn | 250 g | EUR 169.2 |
Sodium chloride (Molecular Biology Grade) |
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| CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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| CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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| CE207 | GeneOn | 5 kg | EUR 123.6 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE224 | GeneOn | 500 g | EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE225 | GeneOn | 1 kg | EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE226 | GeneOn | 5 kg | EUR 207.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE234 | GeneOn | 250 g | EUR 99.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE235 | GeneOn | 500 g | EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE236 | GeneOn | 1 kg | EUR 223.2 |
TritonX-100 (Molecular Biology Grade) |
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| CE240 | GeneOn | 500 ml | EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
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| CE241 | GeneOn | 1 l | EUR 79.2 |
Tween 20, Molecular Biology Grade |
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| T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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| T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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| T9100-100 | GenDepot | 1L | EUR 160.8 |
1L Molecular Biology Grade Water |
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| 46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 177.84 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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| GK8512-1L | Glentham Life Sciences | 1 l | EUR 92.4 |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
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| GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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| PD0252 | Bio Basic | 500g | EUR 192.59 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE135 | GeneOn | 250 g | EUR 72 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE136 | GeneOn | 500 g | EUR 86.4 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE137 | GeneOn | 1 kg | EUR 124.8 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE138 | GeneOn | 5 kg | EUR 418.8 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE148 | GeneOn | 500 g | EUR 67.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE149 | GeneOn | 1 kg | EUR 75.6 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE150 | GeneOn | 5 kg | EUR 180 |
Yeast extract powder (Molecular Biology Grade) |
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| CE169 | GeneOn | 500 g | EUR 133.2 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE174 | GeneOn | 1 g | EUR 232.8 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE175 | GeneOn | 5 g | EUR 920.4 |
Magnesium acetate - Tetrahydrate (Molecular Biology Grade) |
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| CE190 | GeneOn | 500 g | EUR 98.4 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE198 | GeneOn | 1 g | EUR 91.2 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE199 | GeneOn | 5 g | EUR 244.8 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE200 | GeneOn | 250 mg | EUR 92.4 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE201 | GeneOn | 1 g | EUR 190.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE204 | GeneOn | 500 mg | EUR 374.4 |
SSC Buffer (20X) (Molecular Biology Grade) |
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| CE229 | GeneOn | 1 l | EUR 86.4 |
XTT sodium salt (Molecular Biology Grade) |
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| CE250 | GeneOn | 100 mg | EUR 208.8 |
XTT sodium salt (Molecular Biology Grade) |
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| CE251 | GeneOn | 500 mg | EUR 612 |
Tetrahydrofuran |
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| GK1522-1L | Glentham Life Sciences | 1 l | EUR 84 |
Tetrahydrofuran |
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| GK1522-2500ML | Glentham Life Sciences | 2500 ml | EUR 122.4 |
Tetrahydrofuran |
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| S-3460 | Scientific Laboratory Supplies | 1ML | EUR 38.76 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE100 | GeneOn | 50 g | EUR 128.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE101 | GeneOn | 100 g | EUR 193.2 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE102 | GeneOn | 250 g | EUR 387.6 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE103 | GeneOn | 500 g | EUR 656.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE104 | GeneOn | 1 kg | EUR 1162.8 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-193 | Genesee Scientific | 100ml/Unit | EUR 268 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-194 | Genesee Scientific | 500ml/Unit | EUR 294 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-195 | Genesee Scientific | 1000ml/Unit | EUR 308 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-196 | Genesee Scientific | 4 x 1L/Unit | EUR 471 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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| GK0126-1L | Glentham Life Sciences | 1 l | EUR 136.8 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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| GK0126-2500ML | Glentham Life Sciences | 2500 ml | EUR 246 |
Tetrahydrofuran-d8 |
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| S-3461 | Scientific Laboratory Supplies | 1ML | EUR 71.82 |
Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g |
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| 41028-100G | Biotium | 100G | EUR 266.4 |
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Description: Minimum order quantity: 1 unit of 100G |
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Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g |
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| 41028-25G | Biotium | 25G | EUR 130.8 |
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Description: Minimum order quantity: 1 unit of 25G |
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Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g |
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| 41028-500G | Biotium | 500G | EUR 862.8 |
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Description: Minimum order quantity: 1 unit of 500G |
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EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade) |
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| CE141 | GeneOn | 500 ml | EUR 87.6 |
LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
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| CE178 | GeneOn | 500 g | EUR 109.2 |
Ultimately, one of many pathways through which CLEC12A regulates uric acid crystal-stimulated launch of IL-Eight by neutrophils is through a p38/PI3K-Akt signaling pathway. In abstract this work defines early molecular occasions that underpin CLEC12A signaling in human neutrophils to modulate cytokine synthesis. Targeting this pathway could be helpful therapeutically to dampen irritation.
