In this research, the molecular mechanisms through which Mitochondrial Ribosomal Protein S17 (MRPS17) contributes to gastric cancer (GC) and its prognostic significance in GC have been explored. As a protein encoding gene, MRPS17 encodes a 28s proteins belonging the ribosomal protein S17P household. The particular roles and molecular mechanisms of MRPS17 in cancers stay ambiguous. It was revealed by analyzing information from TCGA and GEO that elevated expression of MRPS17 was considerably related to invasion of GC and poor survival of GC sufferers.
Then through univariate and multivariate Cox regression analyses it was demonstrated that MRPS17 an unbiased prognostic issue for GC sufferers (P<0.001). It was demonstrated by differentially expressed gene evaluation and purposeful enrichment evaluation that MPRS17 is expounded to PI3K/AKT pathway and Cell adhesion molecules (CAMs), whereas its operate is mediated by collagen-containing extracellular matrix and receptor ligand/regulator exercise.
Then it was confirmed by in-vitro experiments that pulling down of MRPS17 gene in AGS and SGC7901 cells would considerably inhibit proliferation and invasion functionality of those cells. Furthermore, it was revealed by cell immunofluorescence assay that as a ribosomalprotein, MRPS17 was primarily distributed within the cytoplasmic floor of cell membrane.
Additionally, activation of PI3K/AKT pathway is accountable for malignant development of glioma that was promoted by MRPS17. In conclusion, it was revealed within the current research that MRPS17 promoted invasion and metastasis of GC and potential molecular mechanisms through which it exerted its influences on GC had been explored, suggesting its potential as a novel prognostic biomarker for GC.
Molecular Characterization of Superficial Layers of the Presubiculum During Development
The presubiculum, a subarea of the parahippocampal area, performs a essential function in spatial navigation and spatial illustration. An excellent facet of presubicular spatial codes is head-direction selectivity of the firing of excitatory neurons, known as head-direction cells. Head-direction selectivity emerges earlier than eye-opening in rodents and is maintained in maturity through neurophysiological interactions between excitatory and inhibitory neurons.
Although the presubiculum has been physiologically profiled when it comes to spatial illustration throughout improvement, the histological traits of the growing presubiculum are poorly understood. We discovered that the expression of vesicular glutamate transporter 2 (VGluT2) could be used to delimit the superficial layers of the presubiculum, which was recognized utilizing an anterograde tracer injected into the anterior thalamic nucleus (ATN).
Thus, we immunostained slices from mice ranging in age from neonates to adults utilizing an antibody towards VGluT2 to judge the VGluT2-positive space, which was recognized because the superficial layers of the presubiculum, throughout improvement. We additionally immunostained the slices utilizing antibodies towards parvalbumin (PV) and somatostatin (SOM) and discovered that within the presubicular superficial layers, PV-positive neurons progressively elevated in quantity throughout improvement, whereas SOM-positive neurons exhibited no rising development.
In addition, we noticed repeating patch buildings in presubicular layer III from postnatal days 12. The considerable expression of VGluT2 means that the presubicular superficial layers are regulated primarily by VGluT2-mediated excitatory neurotransmission. Moreover, developmental modifications within the densities of PV- and SOM-positive interneurons and the emergence of the VGluT2-positive patch buildings throughout adolescence could be related to the purposeful improvement of spatial codes within the superficial layers of the presubiculum.
Rice Seedling Growth Promotion by Biochar Varies With Genotypes and Application Dosages
While biochar use in agriculture is broadly advocated, how the impact of biochar on plant development varies with biochar varieties and crop genotypes is poorly addressed. The function of dissolvable natural matter (DOM) in plant development has been more and more addressed for crop manufacturing with biochar. In this research, a hydroponic tradition of rice seedling development of two cultivars was handled with bulk mass (DOM-containing), water extract (DOM solely), and extracted residue (DOM-free) of maize residue biochar, at a volumetric dosage of 0.01, 0.05, and 0.1%, respectively.
On seedling root development of the 2 cultivars, bulk biochar exerted a usually unfavorable impact, whereas the biochar extract had a constantly constructive impact throughout the appliance dosages. Differently, the extracted biochar confirmed a contrasting impact between genotypes. In one other hydroponic tradition with Wuyunjing 7 handled with biochar extract at sequential dosages, seedling development was promoted by 95% at 0.01% dosage however by 26% at 0.1% dosage, defined with the nice promotion of secondary roots slightly than of main roots.
Such results had been possible defined by low molecular weight natural acids and nanoparticles contained within the biochar DOM. This research highlights the significance of biochar DOM and crop genotype when evaluating the impact of biochar on crops. The use of low dosage of biochar DOM could assist farmers to undertake biochar expertise as an answer for agricultural sustainability.
The Inhibitory Receptor CLEC12A Regulates PI3K-Akt Signaling to Inhibit Neutrophil Activation and Cytokine Release
The myeloid inhibitory C-type lectin receptor CLEC12A limits neutrophil activation, pro-inflammatory pathways and illness in mouse fashions of inflammatory arthritis by a molecular mechanism that continues to be poorly understood. We addressed how CLEC12A-mediated inhibitory signaling counteracts activating signaling by cross-linking CLEC12A in human neutrophils. CLEC12A cross-linking induced its translocation to flotillin-rich membrane domains the place its ITIM was phosphorylated in a Src-dependent method.
Phosphoproteomic evaluation recognized candidate signaling molecules regulated by CLEC12A that embrace MAPKs, phosphoinositol kinases and members of the JAK-STAT pathway. Stimulating neutrophils with uric acid crystals, the etiological agent of gout, drove the hyperphosphorylation of p38 and Akt.
Tetrahydrofuran |
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| GK1522-2500ML | Glentham Life Sciences | 2500 ml | EUR 122.4 |
Tetrahydrofuran |
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| GK1522-1 | Glentham Life Sciences | 1 | EUR 39.4 |
Tetrahydrofuran |
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| GK1522-2500 | Glentham Life Sciences | 2500 | EUR 71.1 |
Tetrahydrofuran |
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| GK1522-500 | Glentham Life Sciences | 500 | EUR 23.8 |
Tetrahydrofuran |
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| S-3460 | Scientific Laboratory Supplies | 1ML | EUR 45.6 |
Tetrahydrofuran-d8 |
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| S-3461 | Scientific Laboratory Supplies | 1ML | EUR 84 |
VOC Tetrahydrofuran Neat |
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| REVOC176N | Scientific Laboratory Supplies | 10MG | EUR 46.87 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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| GK0126-1L | Glentham Life Sciences | 1 l | EUR 136.8 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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| GK0126-2500ML | Glentham Life Sciences | 2500 ml | EUR 246 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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| GK0126-1 | Glentham Life Sciences | 1 | EUR 83.1 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
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| GK0126-2500 | Glentham Life Sciences | 2500 | EUR 174 |
Tetrahydrofuran 〈H2O<50ppm〉 |
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| 04113-81 | NACALAI TESQUE | 1L | EUR 64.4 |
Tetrahydrofuran 〈H2O<50ppm〉 |
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| 04113-94 | NACALAI TESQUE | 100ML | EUR 14.7 |
2-Methyl Tetrahydrofuran |
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| 24707-100 | Polysciences Europe GmbH | 100g | EUR 55 |
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Description: 96-47-9 |
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VOC Std Tetrahydrofuran 2000ug/mL in MeOH |
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| REVOC176 | Scientific Laboratory Supplies | 1ML | EUR 66.59 |
Tetrahydrofuran, GlenUltra™, analytical grade, for LC |
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| GS2560-1 | Glentham Life Sciences | 1 | EUR 176.2 |
Tetrahydrofuran, GlenUltra™, analytical grade, for LC |
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| GS2560-2500 | Glentham Life Sciences | 2500 | EUR 294 |
Tetrahydrofuran, GlenDry™, anhydrous |
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| GS6029-1 | Glentham Life Sciences | 1 | EUR 121 |
Tetrahydrofuran, GlenDry™, anhydrous |
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| GS6029-100 | Glentham Life Sciences | 100 | EUR 66.4 |
Tetrahydrofuran, GlenDry™, anhydrous |
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| GS6029-2500 | Glentham Life Sciences | 2500 | EUR 223 |
Tetrahydrofuran, GlenDry™, anhydrous |
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| GS6029-500 | Glentham Life Sciences | 500 | EUR 88.7 |
Cobalt(II) chloride-1,5-Tetrahydrofuran |
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| GX7281-10 | Glentham Life Sciences | 10 | EUR 88.9 |
Cobalt(II) chloride-1,5-Tetrahydrofuran |
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| GX7281-25 | Glentham Life Sciences | 25 | EUR 131.1 |
(R)-2-(Tetrahydrofuran-3-Yl)Acetic-Acid |
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| abx188023-10g | Abbexa | 10 g | EUR 2932.8 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-100ML | Glentham Life Sciences | 100 ml | EUR 93.6 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-1L | Glentham Life Sciences | 1 l | EUR 154.8 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-2500ML | Glentham Life Sciences | 2500 ml | EUR 265.2 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-500ML | Glentham Life Sciences | 500 ml | EUR 117.6 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-1 | Glentham Life Sciences | 1 | EUR 97.7 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-100 | Glentham Life Sciences | 100 | EUR 47.4 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-2500 | Glentham Life Sciences | 2500 | EUR 189.8 |
Tetrahydrofuran, anhydrous, 99.9%, unstabilised |
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| GK8887-500 | Glentham Life Sciences | 500 | EUR 67.2 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE204 | GeneOn | 500 mg | EUR 374.4 |
Tetrahydrofuran, GlenPure™, analytical grade |
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| GS8138-1 | Glentham Life Sciences | 1 | EUR 77.9 |
Tetrahydrofuran, GlenPure™, analytical grade |
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| GS8138-2500 | Glentham Life Sciences | 2500 | EUR 152.7 |
Tetrahydrofuran, GlenPure™, analytical grade |
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| GS8138-500 | Glentham Life Sciences | 500 | EUR 63.5 |
(3R,4S)-Tetrahydrofuran-3,4-diamine dihydrochloride |
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| abx180099-1g | Abbexa | 1 g | EUR 2431.2 |
Urea, suitable for molecular biology |
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| GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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| GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
Urea, suitable for molecular biology |
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| GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
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| GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Tetrahydrofuran, GlenDry™, anhydrous stabilised with BHT |
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| GS2203-1 | Glentham Life Sciences | 1 | EUR 121 |
Tetrahydrofuran, GlenDry™, anhydrous stabilised with BHT |
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| GS2203-100 | Glentham Life Sciences | 100 | EUR 66.4 |
Tetrahydrofuran, GlenDry™, anhydrous stabilised with BHT |
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| GS2203-2500 | Glentham Life Sciences | 2500 | EUR 223 |
Tetrahydrofuran, GlenDry™, anhydrous stabilised with BHT |
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| GS2203-500 | Glentham Life Sciences | 500 | EUR 88.7 |
Sodium chloride, suitable for molecular biology |
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| GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
Sucrose, GlenBiol, suitable for molecular biology |
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| GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Tetrahydrofuran, GlenPure™, analytical grade stabilised with BHT |
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| GS4339-1 | Glentham Life Sciences | 1 | EUR 77.9 |
Tetrahydrofuran, GlenPure™, analytical grade stabilised with BHT |
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| GS4339-2500 | Glentham Life Sciences | 2500 | EUR 152.7 |
Tetrahydrofuran, GlenPure™, analytical grade stabilised with BHT |
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| GS4339-500 | Glentham Life Sciences | 500 | EUR 63.5 |
NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 360 |
DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 243.6 |
Tris (Molecular Biology Grade) |
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| CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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| CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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| CE239 | GeneOn | 5 kg | EUR 535.2 |
BCIP (Molecular Biology Grade) |
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| CE108 | GeneOn | 250 mg | EUR 75.6 |
BCIP (Molecular Biology Grade) |
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| CE109 | GeneOn | 1 g | EUR 108 |
DAPI (Molecular Biology Grade) |
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| CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
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| CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
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| CE119 | GeneOn | 100 mg | EUR 382.8 |
DMSO, Molecular Biology Grade |
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| 40470006-1 | Bio-WORLD | 100 mL | EUR 88.18 |
DMSO, Molecular Biology Grade |
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| 40470006-2 | Bio-WORLD | 250 mL | EUR 150.19 |
DMSO, Molecular Biology Grade |
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| 40470006-3 | Bio-WORLD | 500 mL | EUR 279.26 |
EGTA, Molecular Biology Grade |
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| 40500028-2 | Bio-WORLD | 50 g | EUR 106.43 |
EGTA, Molecular Biology Grade |
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| 40500028-3 | Bio-WORLD | 100 g | EUR 177.58 |
EGTA, Molecular Biology Grade |
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| 40500028-4 | Bio-WORLD | 500 g | EUR 603.19 |
EGTA, Molecular Biology Grade |
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| 40500028-5 | Bio-WORLD | 1 kg | EUR 912.98 |
EGTA, Molecular Biology Grade |
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| 40500028-6 | Bio-WORLD | 2 kg | EUR 1687.94 |
100mL Molecular Biology Grade |
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| 46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 74.4 |
500mL Molecular Biology Grade |
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| 46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 138 |
HEPES (Molecular Biology Grade) |
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| CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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| CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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| CE173 | GeneOn | 1 kg | EUR 424.8 |
Water (Molecular Biology Grade) |
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| CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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| CE244 | GeneOn | 1 l | EUR 67.2 |
CHAPS (Molecular Biology Grade) |
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| CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
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| CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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| CE116 | GeneOn | 25 g | EUR 492 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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| GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
Sucrose, GlenBiol™, suitable for molecular biology |
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| GC3201-1 | Glentham Life Sciences | 1 | EUR 45.1 |
Tween20 (Molecular Biology Grade) |
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| CE242 | GeneOn | 1 l | EUR 106.8 |
Glycine (Molecular Biology Grade) |
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| CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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| CE159 | GeneOn | 5 kg | EUR 228 |
Agarose, Molecular Biology Grade |
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| 40100164-1 | Bio-WORLD | 25 g | Ask for price |
Agarose, Molecular Biology Grade |
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| 40100164-2 | Bio-WORLD | 50 g | Ask for price |
Agarose, Molecular Biology Grade |
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| 40100164-3 | Bio-WORLD | 100 g | Ask for price |
Agarose, Molecular Biology Grade |
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| 40100164-4 | Bio-WORLD | 500 g | Ask for price |
Agarose, Molecular Biology Grade |
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| 40100164-5 | Bio-WORLD | 1 kg | Ask for price |
(S)-4-Iodo-1-Chloro-2-(4-Tetrahydrofuran-3-Yloxy-Benzyl)-Benzene |
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| abx188037-100g | Abbexa | 100 g | EUR 927.6 |
Pyridine, GlenBiol™, suitable for molecular biology |
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| GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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| GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Lysozyme (Molecular Biology Grade) |
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| CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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| CE189 | GeneOn | 10 g | EUR 247.2 |
Formamide, GlenBiol™, suitable for molecular biology |
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| GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
1L Molecular Biology Grade Water |
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| 46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 196.8 |
Tween 20, Molecular Biology Grade |
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| T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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| T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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| T9100-100 | GenDepot | 1L | EUR 160.8 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
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| GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE224 | GeneOn | 500 g | EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE225 | GeneOn | 1 kg | EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE226 | GeneOn | 5 kg | EUR 207.6 |
Boric Acid, Molecular Biology Grade |
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| 40200060-1 | Bio-WORLD | 500 g | EUR 46.16 |
Boric Acid, Molecular Biology Grade |
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| 40200060-2 | Bio-WORLD | 1 kg | EUR 76.66 |
Ultimately, one of many pathways through which CLEC12A regulates uric acid crystal-stimulated launch of IL-Eight by neutrophils is through a p38/PI3K-Akt signaling pathway. In abstract this work defines early molecular occasions that underpin CLEC12A signaling in human neutrophils to modulate cytokine synthesis. Targeting this pathway could be helpful therapeutically to dampen irritation.
