Acetyl-CoA acyltransferase 1 (ACAA1) features as a key regulator of fatty acid β-oxidation in peroxisomes by catalyzing the cleavage of 3-ketoacyl-CoA to acetyl-CoA and acyl-CoA, which take part in the extension and degradation of fatty acids. Thus, ACAA1 is a vital regulator of lipid metabolism and performs a necessary function in fatty acid oxidation and lipid metabolism. Our earlier examine findings revealed that ACAA1 is intently related to the peroxisome proliferator-activated receptor (PPAR) signaling and fatty acid metabolism pathways, that are concerned in fats deposition in sheep, resulting in our speculation that ACAA1 could also be concerned in fats deposition by regulating lipid metabolism.
However, the related molecular mechanism stays unclear. In the current examine, to evaluate the potential operate of ACAA1 in sheep preadipocyte differentiation, we knocked down and overexpressed ACAA1 in sheep preadipocytes and evaluated the sample of ACAA1 gene expression throughout preadipocyte differentiation by qRT-PCR. ACAA1 was considerably expressed in the early stage of adipocyte differentiation, after which its expression decreased.
ACAA1 deficiency elevated lipid accumulation and the triglyceride content material and promoted sheep preadipocyte differentiation, whereas ACAA1 overexpression inhibited adipogenesis and decreased lipid accumulation and the triglyceride content material. Simultaneously, we demonstrated that ACAA1 deficiency upregulated the expressions of the adipogenic marker genes PPARγ and C/EBPα in sheep preadipocytes, however ACAA1 overexpression inhibited the expressions of these markers, indicating that ACAA1 impacts lipid metabolism by regulating adipogenic marker genes. Our outcomes might promote a greater understanding of the regulation of adipogenesis by ACAA1.
The molecular foundation for SARS-CoV-2 binding to canine ACE2
SARS-CoV-2 can infect many home animals, together with canines. Herein, we present that canine angiotensin-converting enzyme 2 (dACE2) can bind to the SARS-CoV-2 spike (S) protein receptor binding area (RBD), and that each pseudotyped and genuine SARS-CoV-2 can infect dACE2-expressing cells. We solved the crystal construction of RBD in advanced with dACE2 and located that the whole quantity of contact residues, contact atoms, hydrogen bonds and salt bridges at the binding interface in this advanced are barely fewer than these in the advanced of the RBD and human ACE2 (hACE2).
This result’s in line with the proven fact that the binding affinity of RBD to dACE2 is decrease than that of hACE2. We additional present that a number of vital mutations in the RBD binding interface play a pivotal function in the binding affinity of RBD to each dACE2 and hACE2. Our work reveals a molecular foundation for cross-species transmission and potential animal unfold of SARS-CoV-2, and offers new clues to dam the potential transmission chains of this virus.
Ependymal cells-CSF circulate regulates stress-induced melancholy
Major depressive dysfunction (MDD) is a extreme, widespread temper dysfunction. While decreased cerebrospinal fluid (CSF) circulate adversely impacts mind metabolism and fluid stability in the growing older inhabitants and through improvement, solely oblique proof hyperlinks aberrant CSF circulation with many ailments together with neurological, neurodegenerative, and psychiatric issues, resembling nervousness and melancholy. Here we present a really excessive focus of p11 as a key molecular determinant for melancholy in ependymal cells, which is considerably decreased in sufferers with MDD, and in two mouse fashions of melancholy induced by continual stress, resembling restraint and social isolation.

The loss of p11 in ependymal cells causes disoriented ependymal planar cell polarity (PCP), decreased CSF circulate, and depression-like and anxiety-like behaviors. p11 intrinsically controls PCP core genes, which mediates CSF circulate. Viral expression of p11 in ependymal cells particularly rescues the pathophysiological and behavioral deficits brought on by loss of p11. Taken collectively, our outcomes determine a brand new function and a key molecular determinant for ependymal cell-driven CSF circulate in temper issues and recommend a novel technique for improvement of therapies for stress-associated neurological, neurodegenerative, and psychiatric issues.
Differential binding of SARS-CoV-2 Spike protein variants to its cognate receptor hACE2 utilizing molecular modeling based mostly binding evaluation
The present emergence of novel coronavirus, SARS-CoV-2 and its ceaseless enlargement worldwide has posed a worldwide well being emergency that has adversely affected the people. With the total world striving to grasp the newly emerged virus, variations in morbidity and an infection fee of SARS-CoV-2 have been noticed throughout diverse geographic areas, which have been ascribed to viral mutation and evolution over time. The homotrimeric Spike (S) glycoprotein on the viral envelope floor is accountable for binding, priming, and initiating an infection in the host.
Our phylogeny evaluation of 1947 sequences of S proteins indicated there’s a change in amino acid (aa) from aspartate (Group-A) to glycine (Group-B) at place 614, close to the receptor- binding area (RBD; aa positions 331-524). The two variants are reported to be in circulation, disproportionately throughout the world, with Group-A dominant in Asia and Group-B in North America.
The trimeric, monomeric, and RBD of S protein of each the variant teams (A & B) have been modeled utilizing the Swiss-Model server and have been docked with the human receptor angiotensin-converting enzyme 2 (hACE2) using the PatchDock server and visualized in PyMol. Group-A S protein’s RBD certain imperceptibly to the two binding clefts of the hACE2 protein, on the different hand, Group-B S protein’s RBD completely interacted inside the binding clefts of hACE2, with larger quantity of hydrogen and hydrophobic interactions.
BCIP (Molecular Biology Grade) |
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CE109 | GeneOn | 1 g | EUR 108 |
CHAPS (Molecular Biology Grade) |
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CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
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CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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CE116 | GeneOn | 25 g | EUR 492 |
DAPI (Molecular Biology Grade) |
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CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
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CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
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CE119 | GeneOn | 100 mg | EUR 382.8 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE121 | GeneOn | 500 ml | EUR 110.4 |
DTT (Molecular Biology Grade) |
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CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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CE133 | GeneOn | 25 g | EUR 243.6 |
Glycine (Molecular Biology Grade) |
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CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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CE159 | GeneOn | 5 kg | EUR 228 |
HEPES (Molecular Biology Grade) |
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CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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CE173 | GeneOn | 1 kg | EUR 424.8 |
Lysozyme (Molecular Biology Grade) |
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CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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CE189 | GeneOn | 10 g | EUR 247.2 |
NAD (Molecular Biology Grade) |
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CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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CE210 | GeneOn | 5 g | EUR 360 |
Tris (Molecular Biology Grade) |
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CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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CE239 | GeneOn | 5 kg | EUR 535.2 |
Tween20 (Molecular Biology Grade) |
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CE242 | GeneOn | 1 l | EUR 106.8 |
Water (Molecular Biology Grade) |
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CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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CE244 | GeneOn | 1 l | EUR 67.2 |
100mL Molecular Biology Grade |
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46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 70.68 |
500mL Molecular Biology Grade |
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46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 124.26 |
Ammonium sulfate (Molecular Biology Grade) |
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CE105 | GeneOn | 250 g | EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
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CE106 | GeneOn | 1 kg | EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
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CE107 | GeneOn | 5 kg | EUR 153.6 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE111 | GeneOn | 250 g | EUR 259.2 |
Formamide deionized (Molecular Biology Grade) |
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CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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CE146 | GeneOn | 1 l | EUR 120 |
Glycerol 87 % (Molecular Biology Grade) |
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CE154 | GeneOn | 1 l | EUR 93.6 |
Glycerol waterfree (Molecular Biology Grade) |
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CE155 | GeneOn | 500 ml | EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
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CE156 | GeneOn | 1 l | EUR 102 |
Glycerol waterfree (Molecular Biology Grade) |
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CE157 | GeneOn | 2.5 l | EUR 170.4 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE160 | GeneOn | 100 g | EUR 93.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE161 | GeneOn | 250 g | EUR 153.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE162 | GeneOn | 500 g | EUR 232.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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CE163 | GeneOn | 1 kg | EUR 352.8 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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CE164 | GeneOn | 100 g | EUR 86.4 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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CE165 | GeneOn | 500 g | EUR 192 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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CE166 | GeneOn | 1 kg | EUR 307.2 |
MOPS buffer (Molecular Biology Grade) |
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CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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CE195 | GeneOn | 250 g | EUR 169.2 |
Sodium chloride (Molecular Biology Grade) |
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CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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CE207 | GeneOn | 5 kg | EUR 123.6 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE224 | GeneOn | 500 g | EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE225 | GeneOn | 1 kg | EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE226 | GeneOn | 5 kg | EUR 207.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE234 | GeneOn | 250 g | EUR 99.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE235 | GeneOn | 500 g | EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE236 | GeneOn | 1 kg | EUR 223.2 |
TritonX-100 (Molecular Biology Grade) |
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CE240 | GeneOn | 500 ml | EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
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CE241 | GeneOn | 1 l | EUR 79.2 |
Water, Ultrapure Molecular Biology Grade |
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41024-4L | Biotium | 4L | EUR 145.2 |
Description: Minimum order quantity: 1 unit of 4L |
Tween 20, Molecular Biology Grade |
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T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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T9100-100 | GenDepot | 1L | EUR 160.8 |
1L Molecular Biology Grade Water |
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46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 177.84 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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GK8512-1L | Glentham Life Sciences | 1 l | EUR 92.4 |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
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GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
Crystalline |
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5-01010 | CHI Scientific | 10mg | Ask for price |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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CE135 | GeneOn | 250 g | EUR 72 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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CE136 | GeneOn | 500 g | EUR 86.4 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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CE137 | GeneOn | 1 kg | EUR 124.8 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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CE138 | GeneOn | 5 kg | EUR 418.8 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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CE148 | GeneOn | 500 g | EUR 67.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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CE149 | GeneOn | 1 kg | EUR 75.6 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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CE150 | GeneOn | 5 kg | EUR 180 |
Yeast extract powder (Molecular Biology Grade) |
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CE169 | GeneOn | 500 g | EUR 133.2 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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CE174 | GeneOn | 1 g | EUR 232.8 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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CE175 | GeneOn | 5 g | EUR 920.4 |
Magnesium acetate - Tetrahydrate (Molecular Biology Grade) |
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CE190 | GeneOn | 500 g | EUR 98.4 |
NADH - Disodium salt (Molecular Biology Grade) |
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CE198 | GeneOn | 1 g | EUR 91.2 |
NADH - Disodium salt (Molecular Biology Grade) |
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CE199 | GeneOn | 5 g | EUR 244.8 |
NADP - sodium salt (Molecular Biology Grade) |
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CE200 | GeneOn | 250 mg | EUR 92.4 |
NADP - sodium salt (Molecular Biology Grade) |
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CE201 | GeneOn | 1 g | EUR 190.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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CE204 | GeneOn | 500 mg | EUR 374.4 |
SSC Buffer (20X) (Molecular Biology Grade) |
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CE229 | GeneOn | 1 l | EUR 86.4 |
XTT sodium salt (Molecular Biology Grade) |
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CE250 | GeneOn | 100 mg | EUR 208.8 |
XTT sodium salt (Molecular Biology Grade) |
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CE251 | GeneOn | 500 mg | EUR 612 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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CE100 | GeneOn | 50 g | EUR 128.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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CE101 | GeneOn | 100 g | EUR 193.2 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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CE102 | GeneOn | 250 g | EUR 387.6 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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CE103 | GeneOn | 500 g | EUR 656.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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CE104 | GeneOn | 1 kg | EUR 1162.8 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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18-193 | Genesee Scientific | 100ml/Unit | EUR 268 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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18-194 | Genesee Scientific | 500ml/Unit | EUR 294 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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18-195 | Genesee Scientific | 1000ml/Unit | EUR 308 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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18-196 | Genesee Scientific | 4 x 1L/Unit | EUR 471 |
EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade) |
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CE141 | GeneOn | 500 ml | EUR 87.6 |
LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
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CE178 | GeneOn | 500 g | EUR 109.2 |
LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
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CE179 | GeneOn | 2.5 kg | EUR 301.2 |
LB-Agar - Powder according to Miller (Molecular Biology Grade) |
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CE180 | GeneOn | 500 g | EUR 104.4 |
LB-Agar - Powder according to Miller (Molecular Biology Grade) |
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CE181 | GeneOn | 2.5 kg | EUR 295.2 |
LB-Medium - Powder according to Lennox (Molecular Biology Grade) |
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CE182 | GeneOn | 500 g | EUR 108 |
LB-Medium - Powder according to Lennox (Molecular Biology Grade) |
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CE183 | GeneOn | 2.5 kg | EUR 301.2 |
LB-Medium - Powder according to Miller (Molecular Biology Grade) |
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CE184 | GeneOn | 2.5 kg | EUR 295.2 |
Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g |
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41028-100G | Biotium | 100G | EUR 266.4 |
Description: Minimum order quantity: 1 unit of 100G |
Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g |
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41028-25G | Biotium | 25G | EUR 130.8 |
Description: Minimum order quantity: 1 unit of 25G |
Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g |
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41028-500G | Biotium | 500G | EUR 862.8 |
Description: Minimum order quantity: 1 unit of 500G |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT |
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AGR-LE-100 | CORNING | 1/pk | EUR 201.6 |
Description: Bioscience Mol Bio; Agarose |
Bulk Beads Zr 0.1mm Triple-Pure Molecular Biology Grade 250g |
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HOM3048 | Scientific Laboratory Supplies | 250G | EUR 536.94 |
Bulk Beads Zr 0.5mm Triple-Pure Molecular Biology Grade 250g |
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HOM3050 | Scientific Laboratory Supplies | 250G | EUR 536.94 |
Bulk Beads Zr 1.0mm Triple-Pure Molecular Biology Grade 250g |
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HOM3052 | Scientific Laboratory Supplies | 250G | EUR 536.94 |
Bulk Beads Zr 1.5mm Triple-Pure Molecular Biology Grade 250g |
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HOM3054 | Scientific Laboratory Supplies | 250G | EUR 536.94 |
Bulk Beads Zr 3.0mm Triple-Pure Molecular Biology Grade 300g |
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HOM3056 | Scientific Laboratory Supplies | 300G | EUR 536.94 |
Phenol |
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20-abx184753 | Abbexa |
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Phenol |
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S-3030 | Scientific Laboratory Supplies | 1ML | EUR 38.76 |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE |
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AGR-LE-5001 | CORNING | 1/pk | EUR 3669.6 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE |
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AGR-MS-1001 | CORNING | 1/pk | EUR 2445.6 |
Description: Bioscience Mol Bio; Agarose |
Hettich Lid For Rotor Phenol Resistant |
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CEN0811 | Scientific Laboratory Supplies | EACH | EUR 282.72 |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE |
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AGR-LE-1001 | CORNING | 1/pk | EUR 926.4 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL) |
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AGR-LM-1001 | CORNING | 1/pk | EUR 4484.4 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL) |
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AGR-LM-501 | CORNING | 1/pk | EUR 2490 |
Description: Bioscience Mol Bio; Agarose |
Crystalline [H-Trp-Gly-OH; MW: 261.2] |
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SP-55342-1 | Alpha Diagnostics | 1 mg | EUR 169.2 |
2 Deoxyadenosine 5Triphosphate Disodium Crystalline dATP 250mg |
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27185004 | Scientific Laboratory Supplies | EACH | EUR 355.68 |
Nonyl Phenol |
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AG037 | Unibiotest | 1 mg | EUR 627.6 |
Water Phenol |
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abx082109-250ml | Abbexa | 250 ml | EUR 226.8 |
Nonyl Phenol |
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AT037 | Unibiotest | 1mg | EUR 1641.6 |
Phenol, 99% |
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GK6990-100G | Glentham Life Sciences | 100 g | EUR 52.8 |
Phenol, 99% |
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GK6990-1KG | Glentham Life Sciences | 1 kg | EUR 93.6 |
Phenol, 99% |
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GK6990-500G | Glentham Life Sciences | 500 g | EUR 67.2 |
Phenol Red |
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GT9844-100G | Glentham Life Sciences | 100 g | EUR 132 |
Phenol Red |
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GT9844-250G | Glentham Life Sciences | 250 g | EUR 217.2 |
Phenol Red |
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GT9844-25G | Glentham Life Sciences | 25 g | EUR 74.4 |
Phenol Red |
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GT9844-5G | Glentham Life Sciences | 5 g | EUR 55.2 |
Phenol red |
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PB0420 | Bio Basic | 25g | EUR 85.06 |
Phenol-d6 |
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S-3035 | Scientific Laboratory Supplies | 1ML | EUR 38.76 |
ELISA kit for Human PST (Phenol Sulfotransferase) |
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ELK3246 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
ELISA kit for Mouse PST (Phenol Sulfotransferase) |
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ELK3642 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
ELISA kit for Rat PST (Phenol Sulfotransferase) |
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ELK6740 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Phenol Std Phenol 2000ug/mL in MeOH |
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REPHE110 | Scientific Laboratory Supplies | 1ML | EUR 88.92 |
Phenol Std Phenol 100ug/mL in DCM |
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REPHE119 | Scientific Laboratory Supplies | 1ML | EUR 43.32 |
This implies that the S protein’s amino acid at place 614 close to the core RBD influences its interplay with the cognate hACE2 receptor, which can induce its infectivity that ought to be explored additional with molecular and biochemical research.